single cell gel electrophoresis meaning in English
单细胞凝胶电泳
Examples
- Protection characteristics of taurine on oxidative damage of human retinal pigment epithelial cells detected by single cell gel electrophoresis
牛磺酸对经单细胞凝胶电泳技术检测人视网膜色素上皮氧化损伤的保护特征 - We applied single cell gel electrophoresis and cell culture technique , which constitute sing cell gel electrophoresis assay system for detecting mutagenicity to detect mutagenesis in vitro induced by animal drug quinocetone and olaquindox . and confirmed optimum lysing - time . vero cells in the period of logarithm - growth time were treated with 9 . 1 ~ 273u mol / l h2o2 at 37 3h , then were lysed for lh , 2h , 3h and 4h to find optimum lysing - time
并基于阳性致突变物h _ 2o _ 2作用于非洲绿猴肾vero细胞引起细胞dna损伤的原理，研究了其关键步骤-裂解时间，以9 . 1 273 mol l剂量的h _ 2o _ 2染毒处于对数生长期的vero细胞3h后，收获细胞用于制备三明治凝胶板，分别裂解1h 、 2h 、 3h和4h并选择最适裂解时间。 - In order to understand the mechanism of mtx further and to investigate the genotoxic target organs , we studied the dna damage and the correlation with dose of mtx by using the alkaline single cell gel electrophoresis ( comet ) assay . liver , spleen , bone marrow , thymus , kidney , testicle , stomach and peripheral lymphocytes of mice were isolated at lh , 3h , 6h , 12h , 24h after 5mg / kg mtx intraperitoneal injection
为了进一步了解甲氨蝶呤( mtx )的作用机制，探测其作用的遗传毒性靶器官，为应用mtx治疗过程中的临床监测和副作用防治提供理论依据，我们以小鼠为研究对象，用单细胞凝胶电泳技术检测了mtx腹腔注射染毒1h 、 3h 、 6h 、 12h 、 24h后对肝、脾、骨髓、胸腺、肾、睾丸、胃和外周血淋巴细胞的dna损伤作用及损伤程度与mtx剂量间的关系。